A current video produced by Vapourtec highlights the patented computerized Variable Mattress Stream Reactor (VBFR) demonstrating absolutely automated strong part peptide synthesis.
Picture Credit score: Vapourtec
The method begins with swelling Tentagel S resin in DMF, preheating and Fmoc deprotection utilizing 20% Piperidine in DMF. A discount in resin quantity marks this deprotection step, tracked on the VBFR’s quantity graph and detected by UV at 365 nm, producing a definite Fmoc peak.
Following deprotection, activated amino acids (AAs) are launched, inflicting the resin quantity to develop. This step is visualized by a darker orange slug travelling by way of the resin. The reagents circulation by way of the resin in a single go solely slightly than conventional recirculation.
Vapourtec’s distinctive single-pass course of offers greater purity crude peptides. The VBFR’s automated system effectively manages repeated cycles of Fmoc deprotection and AA coupling, elongating the peptide chain whereas sustaining exact management over the resin making certain no channeling or dilution of reagents.
The VBFR demonstrates its means to each determine and mitigate aggregation occasions. GLP-1 sequence aggregation is recognized through the coupling of F and E amino acids. By working at elevated temperatures and sustaining a managed surroundings, the method minimizes aggregation severity in comparison with room-temperature or batch strategies.
The Vapourtec software program interface retains customers knowledgeable of every step offering real-time UV and resin quantity information, simplifying the method and permitting researchers to concentrate on optimizing response situations and outcomes with out guide monitoring.”
Victoire Laude, Vapourtec Analysis Scientist
Improved strong part synthesis by automated adjustment of reactor quantityPlay
Video Credit score: Vapourtec Ltd
